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	Provedor de dados Scientia Agricola (1) Genet. Mol. Biol. (1) Autor Simon,Philipp W. (2) Cansev,Asuman (1) Ipek,Ahmet (1) Ipek,Meryem (1) Sahin,Nihan (1) Santos,Carlos A.F. (1) Mais... Palavra-chave Daucus carota (1) Evolution (1) Expressed sequence tags (EST) (1) Genetic relationship (1) Molecular markers (1) SCAR (1) Simple sequence repeat markers (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development and validation of new SSR markers from expressed regions in the garlic genome Scientia Agricola Ipek,Meryem; Sahin,Nihan; Ipek,Ahmet; Cansev,Asuman; Simon,Philipp W.. Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags (EST); Simple sequence repeat markers; Genetic relationship. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100041 Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot Genet. Mol. Biol. Santos,Carlos A.F.; Simon,Philipp W.. Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six... Tipo: Info:eu-repo/semantics/article Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 Registros recuperados: 2 Primeira ... 1 ... Última

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